![]() Furthermore, elevated FST expression in patient tumors is correlated with poor progression-free, post–progression-free, and overall survival. In literature, few studies have been reported using image analysis for the quantification of protein in SDS-PAGE: that is, imaged with Stain-Free technology. such as segmentation of small bones for x-ray densitometry.6 Since the macro. The first time you click inside a mountain, ImageJ will open a new window. ![]() Furthermore, the use of semi-quantitative software as ImageJ (Java-based. FST levels decline to baseline levels in patients no longer receiving chemotherapy with no evidence of disease. The protein purity is generally checked using SDS-PAGE, where densitometry could be used to quantify the protein bands. The macro language of ImageJ is a mix of Pascal and C syntax elements. Click on the magic wand in the ImageJ tool bar, then click once inside each mountain. Due to the technical knowledge required for densitometry analysis together with. Suggesting a role for FST in chemoresistance in patients, FST protein in the abdominal fluid of patients with ovarian cancer significantly increases within 24 hours of chemotherapy exposure. Similarly, CRISPR KO of FST in tumors increased chemotherapy-mediated tumor eradication in an otherwise chemotherapy-resistant tumor model. Consistent with this, CRISPR knockout (KO) of FST in ovarian cancer cells or antibody-mediated neutralization of FST sensitizes ovarian cancer cells to chemotherapy treatment. The ImageJ User Guide guide provides a detailed overview of ImageJ (and inherently Fiji), the standard in scientific image analysis (see 27: Focus on Bioimage Informatics). FST acts in at least a paracrine manner to induce a p-ATF2–dependent quiescent phenotype and chemoresistance in non-quiescent cells. Add 4 volumes of cold acetone to supernatant and incubate 1 h at -20. We found that NFATC4 upregulates FST mRNA and protein expression predominantly in quiescent cells and FST is further upregulated following chemotherapy treatment. Put the powder in tubes, add elution buffer and incubate on a shaker (not sure about appropriate time and temperature).
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